Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0)

CAT#: MRO-961CQ

This antibody is a mouse monoclonal antibody that is capable of binding to HIV-1 p24.

Tested Data
Figure 1 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in ELISA. Figure 2 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in WB. Figure 3 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in Dot Blot. Figure 4 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in SDS-PAGE. Figure 5 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in SEC-HPLC.

Specifications

  • Immunogen
  • HIV-1 p24 protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • HIV-1 p24
  • Species Reactivity
  • HIV-1
  • Clone
  • AG3.0
  • Applications
  • ELISA

Product Property

  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Human immunodeficiency virus type 1; HIV-1; Capsid protein p24; p24
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Breast cancer biomarkers at key points during disease progression Megan Roberts
15.Sep,23
Great for HIV Research
The Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) has been excellent for our HIV research. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
Breast cancer biomarkers at key points during disease progression James Brown
19.Nov,22
Effective in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for HIV-1 p24 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
Breast cancer biomarkers at key points during disease progression Emily Harris
20.Jun,21
Consistent High-Quality Results
This antibody has proven to be consistent and reliable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for HIV-1 p24 have been particularly beneficial.

Q&As

  1. Is the anti-HIV-1 p24 recombinant antibody clone AG3.0 suitable for use in Western blotting?

    A: Yes, the anti-HIV-1 p24 recombinant antibody clone AG3.0 (MRO-961CQ) is suitable for use in Western blotting. It provides specific binding to the HIV-1 p24 protein, allowing for reliable detection in Western blot assays.

  2. What are the storage recommendations for the anti-HIV-1 p24 recombinant antibody clone AG3.0?

    A: The recommended storage condition for the anti-HIV-1 p24 recombinant antibody clone AG3.0 (MRO-961CQ) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

  3. Can the anti-HIV-1 p24 recombinant antibody clone AG3.0 be used in immunoprecipitation assays?

    A: Yes, the anti-HIV-1 p24 recombinant antibody clone AG3.0 (MRO-961CQ) can be used in immunoprecipitation assays. It provides specific binding to the HIV-1 p24 protein, enabling the successful precipitation of the target protein from complex mixtures.

  4. Is the anti-HIV-1 p24 recombinant antibody clone AG3.0 effective in ELISA applications?

    A: Yes, the anti-HIV-1 p24 recombinant antibody clone AG3.0 (MRO-961CQ) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of the HIV-1 p24 protein.

  5. What is the optimal dilution for using the anti-HIV-1 p24 recombinant antibody clone AG3.0 in immunofluorescence?

    A: The optimal dilution for using the anti-HIV-1 p24 recombinant antibody clone AG3.0 (MRO-961CQ) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

  1. Tada, Takuya, et al. "Prophylaxis and treatment of SARS-CoV-2 infection by an ACE2 receptor decoy in a preclinical animal model." Iscience 26.2 (2023). https://doi.org/10.1016/j.isci.2023.106092
    The study conducted by Takuya Tada and colleagues focuses on the development and testing of a high-affinity ACE2 receptor decoy to combat SARS-CoV-2 infection, including variants like Omicron. The decoy, a modified ACE2 ectodomain fused to an immunoglobulin heavy chain Fc region, was shown to significantly suppress virus loads in preclinical animal models. The decoy demonstrated potent neutralizing activity against various SARS-CoV-2 variants, both in vitro and in vivo, by preventing the virus from binding to the ACE2 receptor on host cells. This approach offers a promising prophylactic and therapeutic strategy against current and potentially future SARS-CoV-2 variants, given its ability to target the conserved ACE2 binding mechanism of the virus.
    Creative Biolabs played a crucial role in this study by providing the anti-p24 monoclonal antibody AG3.0 (Cat#: MRO-961CQ). This antibody was essential for the virion-decoy pull-down assay, which involved binding decoy proteins to nickel-nitrilotriacetic acid-agarose beads, incubating with spike protein-pseudotyped virus, and analyzing bound virions on an immunoblot. The anti-p24 antibody AG3.0 enabled the detection of bound virions, facilitating the evaluation of the decoy protein's efficacy in neutralizing the virus. This contribution was significant as it provided a key reagent necessary for the successful execution and analysis of the assay.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# MRO-961CQ, RRID: AB_3111553)

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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